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Accession IconGSE72705

Maternal exposure to a Western-style diet causes differences in microbiota composition and gene expression in the intestine of suckling mouse pups

Organism Icon Mus musculus
Sample Icon 48 Downloadable Samples
Technology Badge Icon Affymetrix Mouse Gene 1.1 ST Array (mogene11st)

Submitter Supplied Information

Description
During the last few decades, the long-lasting consequences of nutritional programming during the early phase of life have become increasingly evident, but the effects of maternal nutrition on the developing intestine are currently still relatively underexplored. In this study, we investigated in mice the effects of a maternal Western-style (WS) high fat/cholesterol diet, given during the perinatal period, on gene expression and microbiota composition of two-week-old offspring. Microarray analysis revealed that a perinatal WS diet caused significant changes in gene expression in the small intestine and colon of the suckling offspring. A strong sexually dimorphic effect was observed in the affected genes. However, pathway analysis of the differentially expressed genes displayed that in both sexes metabolic and immune functions were strongly affected. Integration of the microbiota and gene expression data applying a multivariate correlation analyses revealed that Bacteroidaceae, Porphyromonadaceae and Lachnospiraceae were the bacterial families that most strongly correlated with gene expression in the colon and not with the bacterial families displaying the most pronounced change due to perinatal exposure to a WS diet. Amongst the genes demonstrating a strong correlation with one or more bacterial families were genes of key importance for intestinal development or functioning (i.e., Pitx2 and Ace2). In conclusion, our data demonstrate a strong programming effect of a maternal WS diet on the development of the intestine in the offspring.
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Total Samples
48
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Samples

Show of 48 Total Samples
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colon_female_pup_wsd_replicate6
female
14.000
colon
SI_female_pup_lfd_replicate1
female
14.000
small intestine
SI_male_pup_lfd_replicate4
male
14.000
small intestine
SI_male_pup_lfd_replicate5
male
14.000
small intestine
SI_male_pup_lfd_replicate2
male
14.000
small intestine
colon_male_pup_lfd_replicate5
male
14.000
colon
colon_female_pup_wsd_replicate4
female
14.000
colon
SI_male_pup_wsd_replicate3
male
14.000
small intestine
colon_male_pup_wsd_replicate3
male
14.000
colon
colon_male_pup_wsd_replicate1
male
14.000
colon
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