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Accession IconSRP140095

Inhibition of EGFR signaling downregulates K-RAS mutated activity

Organism Icon Mus musculus
Sample Icon 9 Downloadable Samples
Technology Badge IconIllumina HiSeq 2000

Submitter Supplied Information

Description
KRAS mutations are the ost abundand driver mutations found in lung adenocarcinoma patients. Unfortunately, there are no clinical approved inhibitors available, to directly target mutant forms of KRAS. The aim of the study was to unravel the impact of upstream Egfr activation in signaling of mutated K-ras. We found that upregulation of G12D mutant Kras induced genes was significantly impaired when Egfr was knocked out. Our data suggests that signaling of mutant Kras depends on upstream activation. This finding may be exploited therapeutically by targeting EGFR in KRAS mutant patients. Overall design: We isolated mouse alveolar type II cells and induced the Kras G12D mutation, with and without concomitant Egfr knockout, in vitro. Cells lysates were analyzed 5 days following transgene induction.
PubMed ID
Total Samples
9
Submitter’s Institution
No associated institution

Samples

Show of 9 Total Samples
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Specimen part
Cell line
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Additional Metadata
wt_1
alveolar type ii cells
c57bl/6n
lung
NA
wt_2
alveolar type ii cells
c57bl/6n
lung
NA
wt_3
alveolar type ii cells
c57bl/6n
lung
NA
Khet_3
alveolar type ii cells
c57bl/6n
lung
NA
Khet_Eko2
alveolar type ii cells
c57bl/6n
lung
NA
Khet_Eko3
alveolar type ii cells
c57bl/6n
lung
NA
Khet_1
alveolar type ii cells
c57bl/6n
lung
NA
Khet_2
alveolar type ii cells
c57bl/6n
lung
NA
Khet_Eko1
alveolar type ii cells
c57bl/6n
lung
NA
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