Description

Peripheral blood lymphocytes were separated in the Ficoll gradient and subjected for stimulation with anti-CD3 and anti-CD28 antiobodies upon time (6h, 12h and 18h). Next, total RNA was isolated and trenscriptional analysis of stimulated cells was performed.

Publication Title

Loss-of-function mutations in the IL-21 receptor gene cause a primary immunodeficiency syndrome.

Sample Metadata Fields

Time