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Homo sapiens
6 Downloadable Samples
IlluminaHiSeq2500
Description
Hypoxia plays important roles in progression of chronic kidney diseases. HIF1 (hypoxia inducible factor 1) is a master transcriptional factor under hypoxic condition. To clarify the molecular mechanisms of HIF1 and identify novel linc RNAs under hypoxia, we performed RNA-seq using human renal proximal tubular epithelial cells (RPTEC). In addition, we use Dznep which is an inhibitor of H3K27me3 to examine the relationship between HIF1 and epigenetic modifiers under hypoxia.
Publication Title
No associated publication
Sample Metadata Fields
No sample metadata fields
View Samples- Homo sapiens
- 6 Downloadable Samples
- IlluminaHiSeq2500
Description
Hypoxia plays important roles in progression of chronic kidney diseases. HIF1 (hypoxia inducible factor 1) is a master transcriptional factor under hypoxic condition. To clarify the molecular mechanisms of HIF1 and identify novel lincRNAs under hypoxia, we performed RNA-seq using human renal proximal tubular cells (HK2: human kidney-2). In addition, we use Dznep which is an inhibitor of H3K27me3 to examine the relationship between HIF1 and epigenetic modifiers under hypoxia.
Publication Title
No associated publication
Sample Metadata Fields
No sample metadata fields
View Samples- Mus musculus
- 11 Downloadable Samples
Affymetrix Mouse Gene 2.0 ST Array (mogene20st)
Description
High serum concentrations of kidney-derived protein uromodulin (Tamm-Horsfall protein or THP) have recently been shown to be independently associated with low mortality in both older adults and cardiac patients, but the underlying mechanism remains unclear. Here, we show that THP inhibits the generation of reactive oxygen species (ROS) both in the kidney and systemically. Consistent with this experimental data, the concentration of circulating THP in patients with surgery-induced acute kidney injury (AKI) correlated with systemic oxidative damage. THP in the serum dropped after AKI, and was associated with an increase in systemic ROS. The increase in oxidant injury correlated with post-surgical mortality and need for dialysis. Mechanistically, THP inhibited the activation of the TRPM2 channel. Furthermore, inhibition of TRPM2 in vivo in a mouse model, mitigated the systemic increase in ROS during AKI and THP deficiency. Our results suggest that THP is a key regulator of systemic oxidative stress by suppressing TRPM2 activity and our findings might help to explain how circulating THP deficiency is linked with poor outcomes and increased mortality.
Publication Title
Circulating uromodulin inhibits systemic oxidative stress by inactivating the TRPM2 channel.
Sample Metadata Fields
Specimen part
View Samples- Mus musculus
- 8 Downloadable Samples
- Illumina HiSeq 2500
Description
In chronic kidney disease (CKD) state, various changes in gene expression in kidneys are occurring, reflecting accumulation of uremic toxins, local inflammation, increased oxidative stress, and up-regulation of pro-fibrotic signals. However, little studies have been conducted for comprehensive evaluation of transcriptomic changes in CKD kidneys. Identification of key signals in CKD may lead to the elucidation of the mechanism of CKD progression and the development of therapeutic methods.
Publication Title
No associated publication
Sample Metadata Fields
No sample metadata fields
View Samples
SRP052732- Homo sapiens
- 4 Downloadable Samples
- IlluminaHiSeq2000
Description
Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting
Publication Title
No associated publication
Sample Metadata Fields
No sample metadata fields
View Samples SRP168448- Mus musculus
- 12 Downloadable Samples
- Illumina HiSeq 3000
Description
The expression of lncRNAs in the hypothalamic neuronal stem cells of young mice and aged mice.The expression pattern of mRNAs in the hypothalamic neuronal stem cells of aged Hnscr null mice and littermate wild-type mice.
Publication Title
No associated publication
Sample Metadata Fields
Sex, Specimen part, Cell line, Treatment
View Samples- Mus musculus
- 9 Downloadable Samples
- Illumina HiSeq 4000
Description
To get a deeper understanding of molecular mechanism elicited by alogliptin intervention, high-throughput RNA sequencing (RNA-seq) of the expression levels of all genes in the liver was performed
Publication Title
No associated publication
Sample Metadata Fields
Sex, Specimen part
View Samples- Homo sapiens
- 12 Downloadable Samples
- Affymetrix Human Genome U133 Plus 2.0 Array (hgu133plus2)
Description
This SuperSeries is composed of the SubSeries listed below.
Publication Title
Recurrent mutations in multiple components of the cohesin complex in myeloid neoplasms.
Sample Metadata Fields
Specimen part, Disease, Cell line
View Samples- Homo sapiens
- 4 Downloadable Samples
- Affymetrix Human Genome U133 Plus 2.0 Array (hgu133plus2)
Description
This SuperSeries is composed of the SubSeries listed below.
Publication Title
Classification of Epstein-Barr virus-positive gastric cancers by definition of DNA methylation epigenotypes.
Sample Metadata Fields
Specimen part, Cell line
View Samples- Homo sapiens
- 82 Downloadable Samples
- Affymetrix Human Genome U133 Plus 2.0 Array (hgu133plus2)
Description
The aim of this study is to identify responders to FOLFOX therapy by applying the Random Forests (RF) algorithm to gene expression data. Eighty-three unresectable colorectal cancer (CRC) patients including 42 responders and 41 non-responders were divided into training (54 patients) and test (29 patients) sets.
Publication Title
Potential responders to FOLFOX therapy for colorectal cancer by Random Forests analysis.
Sample Metadata Fields
Sex
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